Rhode Island Science and Technology Advisory Council (STAC)

Making Innovation Central to Rhode Island's Future Gina M. Raimondo, Governor

Last Updated
Jun 3, 2008

OmniLog Phenotype Microarray System

Details

Biolog, Inc. has developed a proprietary technology called Phenotype MicroArrays (PMs). PMs are sets of 96-well microtiter plates with each well containing a different cell culture medium that is designed to test a unique phenotype or cell function. After inoculation with a cell suspension, the plates are placed in the OmniLog incubator-reader, typically for 24 to 48 hours, and the phenotypic behavior is recorded by a CCD camera. The response of the cells in each well is monitored colorimetrically using a patented redox chemistry that integrates the respiration of the cells over time. The result is a color change, from colorless to purple. If the cells display an expected phenotype and can grow in a well, they respire normally to give a dark purple color. If the phenotype and growth are weak, they respire more slowly and give a light purple color. If the phenotype and growth are negative, the well remains colorless. The current PM technology is developed for use with diverse microbial cells including fungi as well as bacteria.

Specifications:

  • OmniLog incubator capacity is 50 standard microplates or PM plates (aka: panels)
  • Incubation Temperature Range: 22° to 45°C in the tray chamber at full capacity
  • Phenotype MicroArray (PM) panels of up to 1,920 phenotypic tests for Gram-negative and Gram-positive bacteria, yeast and filamentous fungi, and mammalian cells
  • OmniLog PM software contains a suite of algorithms that work in conjunction with the OmniLog reader and Biolog’s PM panels. These programs allow display of kinetic PM panel data recorded by the OmniLog reader, manage and analyze the data, export it in a variety of raw and processed forms.
  • The OmniLog® PM System generates a kinetic response curve for all assays simultaneously and monitors most known aspects of cell function. The range of phenotypes analyzed includes:
    1. Nutrient uptake and transport functions
    2. Catabolism of carbon, nitrogen, phosphorus, and sulfur
    3. Biosynthesis of small molecules
    4. Biosynthesis of polymeric macromolecules
    5. Formation of cellular structures
    6. Cellular respiratory functions

Contact

RI Genomics and Sequencing Center at gsc@etal.uri.edu or (401) 874-5919

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