Rhode Island Science and Technology Advisory Council (STAC)

Making Innovation Central to Rhode Island's Future Gina M. Raimondo, Governor

Last Updated
Jun 3, 2008

Strategene Mx4000 Real-Time PCR System

Details

Real-time or quantitative PCR (QPCR) allows researchers to quickly and easily quantify nucleic acids for studying gene expression, mutational analysis, disease state, and gene dosage. QPCR measures PCR product accumulation during the exponential phase of the reaction and before amplification becomes vulnerable to limiting reagents and cycling variability. Fluorescent QPCR data provides accurate information on initial starting copy number. Using QPCR, amplification and detection are combined in a single step and in a single closed tube. This eliminates the need for numerous post-PCR manual steps, and reduces the possibility of introducing variability or laboratory contamination. The Mx4000 system has an open format that allows closed-tube real-time PCR detection with many chemistries including SYBR Green dye and fluorogenic probe systems, detecting up to four different dyes simultaneously. The Mx4000 system supports both real-time and plate read experiments.

Contact

RI Genomics and Sequencing Center at gsc@etal.uri.edu or (401) 874-5919

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