Rhode Island Science and Technology Advisory Council (STAC)

Making Innovation Central to Rhode Island's Future Gina M. Raimondo, Governor

Last Updated
Sep 10, 2008

Genetic Analyzer ABI 3130

Details

This instrument is a Genetic Analyzer ABI 3130 machine with a 4-capillary array. Fully automated from polymer loading and replacement to DNA separation, detection and data analysis enabling 24-hour unattended operation.

Specifications:

  • Autoloading of samples performed from one microtiter plate, either 96- or 384-well format
  • Arrays with four capillaries, currently in two lengths 36, 50.
  • Automated delivery system for POP-4™, POP-6™, or POP-7™ polymers
  • Argon laser with primary excitations at 488 and 515 nm * Detection of up to 5 dyes for sequencing and fragment analysis (microsatellite analysis, AFLP, LOH, SNP validation and SNP screening)
  • CCD detection technology and spectrograph for color separation
  • Software for DNA sequencing/base calling (Sequencing Analysis v5.2), for fragment analysis of microsatellites, SNP, AFLP and LOH (GeneMapper® v4.0), and Variant Reporter Software for reference based and non-reference based analysis

Contact

Elisabeth Arevalo at earevalo@providence.edu or Providence College's Biology Lab at (401) 865-1604 (or 1603)

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