Rhode Island Science and Technology Advisory Council (STAC)

Making Innovation Central to Rhode Island's Future Gina M. Raimondo, Governor

Last Updated
Jun 3, 2008

Biacore T-100 SPR


Surface Plasmon Resonance (SPR), a phenomenon happening when light gets reflected on very thin metal films, is used to investigate protein:protein and protein:ligand interactions. On the backside of the metallic film one protein or ligand can be localized. If a ligand (protein, small molecule) in a mobile phase interacts with the immobilized protein the local reflective index of the gold surface changes. These changes can be detected in real time and are summarized in a sensorgram. The SPR rates can be translated in association rates for the protein:protein or protein:ligand interaction.

The Biacore T-100 instrument enables the rapid determination of binding constants, binding kinetics and thermodynamic parameters of the binding events. Critically, very small quantities of proteins are used for these studies.


RI Proteomics Center, James Clifton, PhD at James_Clifton@brown.edu or (401) 863-7095

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