Rhode Island Science and Technology Advisory Council (STAC)

Making Innovation Central to Rhode Island's Future Gina M. Raimondo, Governor

Last Updated
Jun 3, 2008

Applied Biosystems 3130xl Genetic Analyzer

Details

This instrument is a fluorescence-based genetic analysis system. It is fully automated from polymer loading and replacement, to DNA separation, detection, and data analysis enabling 24-hour unattended operation.

Specifications:

  • Autoloading of samples performed from two microtiter plates; either 96- or 384-well format
  • Arrays with sixteen capillaries in four different lengths: 22, 36, 50, and 80 cm.
  • Automated delivery system for POP-4™, POP-6™, or POP-7™ polymers
  • Argon laser with primary excitations at 488 and 515 nm * Detection of up to 5 dyes for sequencing and fragment analysis
  • CCD detection technology and spectrograph for color separation
  • Simultaneous dual-side illumination detection system to maximize signal uniformity and sensitivity
  • Software for DNA sequencing/base calling (Sequencing Analysis v5.2) and for fragment analysis of microsatellites, SNP, AFLP and LOH (GeneMapper® v4.0) as well as comparative sequencing, mutation detection, and detection of heterozygote insertions and deletions (SeqScape® v2.5)

Contact

RI Genomics and Sequencing Center at gsc@etal.uri.edu or (401) 874-5919

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